Identification of Self-incompatibility Genotypes in Apple and Crabapple Cultivars by S-allele Specific PCR Analysis
نویسندگان
چکیده
منابع مشابه
identification of self-incompatibility (s-) genotypes of iranian almond genotypes and cultivars using pcr
almond (prunus dulcis [webb] d.a. mill) is an economically important species of genus prunus (rosaceae, subfamily prunoideae). the commercial edible part of almond fruit is kernel. fertilization is essential for nut production in almond but most cultivars are self or even cross-incompatible. self-incompatibility in almond is gametophytic and controlled by a single s-locus with multiple codomina...
متن کاملThe Study of Morphological Traits and Identification of Self-incompatibility Alleles in Almond Cultivars and Genotypes
The evaluation of an almond collection using morphological variables and identification of self-incompatibility genotype is useful for selecting pollinizers and for the design of crossing in almond breeding programs. In this study, important morphological traits and self-incompatibilities in 71 almond cultivars and genotypes were studied. Simple and multiplex specific PCR analyses were used in...
متن کاملIdentification of Self- incompatibility Alleles in Some Almond Genotypes by Degenerate S-RNase Primers
The almond, Prunus dulcis Miller which belongs to Rosaceae family, is one of the most important commercial and oldest cultivated tree nut crops. Almonds are classified as a ‘nut’ in which the edible seed is the commercial product. Therefore, pollination and fertilization are necessary in almond. The characteristic of cultivated almond to express gametophytic self- incompatibility discourages se...
متن کاملThe RT-PCR identification and sequence analysis of Apple chlorotic leaf spot virus from apple cultivars in Jiaodong Peninsula, China
A set of specific primer pairs was utilized to detect Apple chlorotic leaf spot virus (ACLSV) from seven different apple cultivars in Jiaodong Peninsula via reverse transcription polymerase chain reaction (RT-PCR), and the sequence of ACLSV genome was analysed. The results indicate that: (1) High-purity total RNA could be successfully isolated using plant RNA rapid extraction kit. The ratios of...
متن کاملdetermination of self-compatible almond genotypes obtained from controlled crosses and identification of s-alleles in some foreign cultivars and genotypes by pcr
self-incompatibility is one of the main problems in almond production. in this research work, at the first step, some local cultivars as well as some imported ones were evaluated from the point of their male and female organ compatibility. then, ten selected commercial self-incompatible cultivars(female parents) were crossed with two self-compatible cultivars(male parents) i. e. tuono and super...
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ژورنال
عنوان ژورنال: Korean Journal of Breeding Science
سال: 2014
ISSN: 2287-5174
DOI: 10.9787/kjbs.2014.46.4.364